E-ISSN 2146-9369 | ISSN 2146-3158
 

Research Article


Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates

Ivy Jepkurui Mutai, Martin Georges, Atunga Nyachieo, Eric Odoyo, Anthony Kebira Nyamache, Jillani Ngalla.


Abstract
Objectives:
The use of bacteriophages (phages) as an alternative treatment for multidrug-resistant bacteria has recently gained popularity. Phage cocktails have been proposed for broad-spectrum therapeutic effects against such resistant bacteria. However, the effectiveness of non-locally formulated phage cocktails for therapy on Kenyan isolates has yet to be evaluated and is a subject of investigation. This study aimed to determine the invitro effectiveness of a non-locally made commercial pyophage cocktail on clinical Pseudomonas aeruginosa isolates from Kenya.
Methods:
Forty-nine P. aeruginosa isolates from Kenya were subjected to a pyophage cocktail for efficacy studies using direct spot test (DST) and efficiency of plating (EOP).
Results:
The success rate of the cocktail was observed on 16.3% (8/49) isolates only and ineffective on 83.7% (41/49) isolates. Of the eight isolates that showed cross-reactivity from DST, six had complete lysis with a faintly hazy background. Five of these six isolates resulted in successful and high phage progeny production in plaquing efficiency (EOP ≥ 0.5).
Conclusion:
Non-locally made commercial pyophage cocktail was ineffective against the 83.7% endemic clinical strains of the Kenyan P. aeruginosa isolates, demonstrating the importance of locally derived phage cocktails against endemic and multidrug-resistant isolates.

Key words: Pyophage cocktail, P. aeruginosa, spot test, the efficiency of plating, host range


 
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How to Cite this Article
Pubmed Style

Mutai IJ, Georges M, Nyachieo A, Odoyo E, Nyamache AK, Ngalla J. Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates. J Microbiol Infect Dis. 2023; 13(1): 25-30. doi:10.5799/jmid.1265378


Web Style

Mutai IJ, Georges M, Nyachieo A, Odoyo E, Nyamache AK, Ngalla J. Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates. https://www.jmidonline.org/?mno=302657062 [Access: April 30, 2024]. doi:10.5799/jmid.1265378


AMA (American Medical Association) Style

Mutai IJ, Georges M, Nyachieo A, Odoyo E, Nyamache AK, Ngalla J. Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates. J Microbiol Infect Dis. 2023; 13(1): 25-30. doi:10.5799/jmid.1265378



Vancouver/ICMJE Style

Mutai IJ, Georges M, Nyachieo A, Odoyo E, Nyamache AK, Ngalla J. Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates. J Microbiol Infect Dis. (2023), [cited April 30, 2024]; 13(1): 25-30. doi:10.5799/jmid.1265378



Harvard Style

Mutai, I. J., Georges, . M., Nyachieo, . A., Odoyo, . E., Nyamache, . A. K. & Ngalla, . J. (2023) Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates. J Microbiol Infect Dis, 13 (1), 25-30. doi:10.5799/jmid.1265378



Turabian Style

Mutai, Ivy Jepkurui, Martin Georges, Atunga Nyachieo, Eric Odoyo, Anthony Kebira Nyamache, and Jillani Ngalla. 2023. Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates. Journal of Microbiology and Infectious Diseases, 13 (1), 25-30. doi:10.5799/jmid.1265378



Chicago Style

Mutai, Ivy Jepkurui, Martin Georges, Atunga Nyachieo, Eric Odoyo, Anthony Kebira Nyamache, and Jillani Ngalla. "Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates." Journal of Microbiology and Infectious Diseases 13 (2023), 25-30. doi:10.5799/jmid.1265378



MLA (The Modern Language Association) Style

Mutai, Ivy Jepkurui, Martin Georges, Atunga Nyachieo, Eric Odoyo, Anthony Kebira Nyamache, and Jillani Ngalla. "Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates." Journal of Microbiology and Infectious Diseases 13.1 (2023), 25-30. Print. doi:10.5799/jmid.1265378



APA (American Psychological Association) Style

Mutai, I. J., Georges, . M., Nyachieo, . A., Odoyo, . E., Nyamache, . A. K. & Ngalla, . J. (2023) Evaluating the effectiveness of a non-locally developed commercial phage cocktail on Kenyan Pseudomonas aeruginosa isolates. Journal of Microbiology and Infectious Diseases, 13 (1), 25-30. doi:10.5799/jmid.1265378