E-ISSN 2146-9369 | ISSN 2146-3158
 

Research Article


Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water

Ashna Bhasin, Manisha Jain, Bibhabati Mishra, Poonam Loomba, Abha Sharma, Tanvi Aggrawal.


Abstract
Objectives:
Providing safe drinking water is essential for maintaining healthcare quality. The presence of biofilms in the water supply protects the organism from the antimicrobial effects of disinfectants leading to the formation of the MDR pathogen pool. Therefore, this study was taken up to determine the prevalence of biofilm formation in the bacteria isolated from the water system of a tertiary health care setup and study the effect of disinfectants on biofilms.
Methods:
Thirty-four drinking water samples were collected in sterile glass stopper bottles and transported to the lab. Standard bacteriological procedures identified isolates. Biofilm detection was carried out by the tissue culture plate (TCP) method. The effect of disinfectant (sodium hypochlorite) at various concentrations (0, 0.25, 0.5, 1, 2, and 4 %) on biofilm-producing organism were studied for 30 minutes and analyzed.
Results:
The culture positivity was 76.4% (26/34). Twenty samples showed monomicrobial growth, while only six samples showed polymicrobial growth of organisms. The most common organism isolated was Pseudomonas aeruginosa. Biofilm production was seen more in polymicrobial organisms, 91.66 % (11/12). A high level of resistance to chlorine compounds was seen in biofilm-producing microorganisms, especially those that produced robust biofilms.
Conclusion:
Resistance of biofilms against high levels of chlorine has implications for the delivery of safe drinking water. Drug resistance was seen in these organisms, which can be transmitted from drinking water sources to humans. Therefore, it is recommended that biofilm production should be evaluated in drinking water samples regularly.

Key words: Biofilm, sodium hypochlorite, drinking water


 
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How to Cite this Article
Pubmed Style

Bhasin A, Jain M, Mishra B, Loomba P, Sharma A, Aggrawal T. Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water. J Microbiol Infect Dis. 2023; 13(1): 17-24. doi:10.5799/jmid.1265969


Web Style

Bhasin A, Jain M, Mishra B, Loomba P, Sharma A, Aggrawal T. Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water. https://www.jmidonline.org/?mno=302657061 [Access: April 30, 2024]. doi:10.5799/jmid.1265969


AMA (American Medical Association) Style

Bhasin A, Jain M, Mishra B, Loomba P, Sharma A, Aggrawal T. Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water. J Microbiol Infect Dis. 2023; 13(1): 17-24. doi:10.5799/jmid.1265969



Vancouver/ICMJE Style

Bhasin A, Jain M, Mishra B, Loomba P, Sharma A, Aggrawal T. Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water. J Microbiol Infect Dis. (2023), [cited April 30, 2024]; 13(1): 17-24. doi:10.5799/jmid.1265969



Harvard Style

Bhasin, A., Jain, . M., Mishra, . B., Loomba, . P., Sharma, . A. & Aggrawal, . T. (2023) Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water. J Microbiol Infect Dis, 13 (1), 17-24. doi:10.5799/jmid.1265969



Turabian Style

Bhasin, Ashna, Manisha Jain, Bibhabati Mishra, Poonam Loomba, Abha Sharma, and Tanvi Aggrawal. 2023. Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water. Journal of Microbiology and Infectious Diseases, 13 (1), 17-24. doi:10.5799/jmid.1265969



Chicago Style

Bhasin, Ashna, Manisha Jain, Bibhabati Mishra, Poonam Loomba, Abha Sharma, and Tanvi Aggrawal. "Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water." Journal of Microbiology and Infectious Diseases 13 (2023), 17-24. doi:10.5799/jmid.1265969



MLA (The Modern Language Association) Style

Bhasin, Ashna, Manisha Jain, Bibhabati Mishra, Poonam Loomba, Abha Sharma, and Tanvi Aggrawal. "Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water." Journal of Microbiology and Infectious Diseases 13.1 (2023), 17-24. Print. doi:10.5799/jmid.1265969



APA (American Psychological Association) Style

Bhasin, A., Jain, . M., Mishra, . B., Loomba, . P., Sharma, . A. & Aggrawal, . T. (2023) Effect of Sodium Hypochlorite on Biofilm Producing Organisms Isolated from A Hospital Drinking Water. Journal of Microbiology and Infectious Diseases, 13 (1), 17-24. doi:10.5799/jmid.1265969