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Research Article 


Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis

Pooja Saxena, Ashish K. Asthana, Molly Madan.


Cited By:1

Abstract
Objective: Tuberculosis (TB) one of the most important major causes of mortality and morbidity around the world. Early detection of TB affords to prevent transmission of TB and better treatment options. The aim of this study is to evaluate sensitivity of fluorescent, Ziehl Neelsen microscopy and Polymerase Chain Reaction (PCR) method.
Methods: Fifty samples of clinically suspected cases of pulmonary tuberculosis were collected and processed for Myco¬bacteria by Fluorescent and ZN staining. Negative samples by staining methods were processed by PCR method using primer IS6110 of M. tuberculosis complex.
Results: 33/50 (66.0%) samples were positive by Fluorescent and 23/50 (46%) were positive by ZN method. All micro¬scopically negative samples (n=17) were processed to PCR and only seven of samples (41.1%) showed positive results.
Conclusion: We concluded that PCR is best but it required lot of investment. J Microbiol Infect Dis 2014; 4(4): 141-144

Key words: Mycobacterium tuberculosis, PCR, fluorescent, Ziehl Neelsen, microscopy, detection. Pooja Saxena, Ashish K. Asthana, Molly Madan. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbiol Infect Dis 2014; 4(4): 141-144

doi: 10.5799/ahinjs.02.2014.04.0157


 
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How to Cite this Article
Pubmed Style

Saxena P, Asthana AK, Madan M. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbiol Infect Dis. 2014; 4(4): 141-144 .

doi:10.5799/jmid2014.04.0157

Web Style

Saxena P, Asthana AK, Madan M. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. https://www.jmidonline.org/?mno=302657129 [Access: June 27, 2026].

doi:10.5799/jmid2014.04.0157

AMA (American Medical Association) Style

Saxena P, Asthana AK, Madan M. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbiol Infect Dis. 2014; 4(4): 141-144 .


doi:10.5799/jmid2014.04.0157

Vancouver/ICMJE Style

Saxena P, Asthana AK, Madan M. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbiol Infect Dis. (2014), [cited June 27, 2026]; 4(4): 141-144 .


doi:10.5799/jmid2014.04.0157

Harvard Style

Saxena, P., Asthana, . A. K. & Madan, . M. (2014) Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. J Microbiol Infect Dis, 4 (4), 141-144 .


doi:10.5799/jmid2014.04.0157

Turabian Style

Saxena, Pooja, Ashish K. Asthana, and Molly Madan. 2014. Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. Journal of Microbiology and Infectious Diseases, 4 (4), 141-144 .


doi:10.5799/jmid2014.04.0157

Chicago Style

Saxena, Pooja, Ashish K. Asthana, and Molly Madan. "Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis." Journal of Microbiology and Infectious Diseases 4 (2014), 141-144 .


doi:10.5799/jmid2014.04.0157

MLA (The Modern Language Association) Style

Saxena, Pooja, Ashish K. Asthana, and Molly Madan. "Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis." Journal of Microbiology and Infectious Diseases 4.4 (2014), 141-144 . Print.


doi:10.5799/jmid2014.04.0157

APA (American Psychological Association) Style

Saxena, P., Asthana, . A. K. & Madan, . M. (2014) Comparison of microscopy and PCR in detection of Mycobacterium tuberculosis. Journal of Microbiology and Infectious Diseases, 4 (4), 141-144 .


doi:10.5799/jmid2014.04.0157

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