Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Mustafa Altındiş; Zafer Çetinkaya; Raike Kalaycı; İhsan H. Çiftçi; Alpaslan Arslan; Orhan C. Aktepe

doi:10.5799/jmid2011.01.0002

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J Microbiol Infect Dis. 2011; 1(1): 5-9

doi: 10.5799/jmid2011.01.0002

Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Mustafa Altındiş, Zafer Çetinkaya, Raike Kalaycı, İhsan H. Çiftçi, Alpaslan Arslan, Orhan C. Aktepe.

Cited By:5

Abstract
Objectives: The aim of the present study was to evaluate the efficacy (recovery rate, time to detection and Drug Susceptibility Tests –DST- of Mycobacteria-only B460) of new colorimetric medium, Dio-TK and to compare it with routinely used conventional media, Lowenstein Jensen (LJ) and Bactec 460 TB culture system.
Materials and methods: Totally 901 clinic specimens were investigated for assignment of tuberculosis by Ehrlich-Ziehl-Nielsen smear strain method, Lowenstein-Jensen, BACTEC 460TB and Dio-TK medium culture systems.
Results: Nineteen of 901 clinic specimens (2.1%) were positive by any of these methods. 17 (89.5%) of these specimens positive found by smear strain method, 17 (89.5%) by Lowenstein-Jensen, 19 (100%) by BACTEC 460TB and 14 (73.7%) by Dio-TK medium. NAP and Niacin identification tests were applied to Mycobacterium strains. 12 (63.1%) of 19 isolates were identified as M.tuberculosis complex and 7 (36.9%) were identified as Mycobacterium other than tuberculosis (MOTT) bacilli. 10 (83.3%) of 12 M.tuberculosis complex strains were not resistant to any major drug. But one of 2 isolate was resistant to streptomycin and the other one isolate was resistant to both streptomycin and isoniazid.
Conclusion: Our data suggest that some advantages (such as an early detection and differentiation mycobacterium growth from contamination) of the Dio-TK CS over other mycobacterial culture systems make it a practical and rapid system for daily use, and a suitable alternative to other currently available solid media, such as LJ, for detection time of mycobacteria and DST. J Microbiol Infect Dis 2011;1(1):5-9.

doi: 10.5799/ahinjs.02.2011.01.0002

Key words: Dio-TK medium, Mycobacterium tuberculosis, detection, resistance

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How to Cite this Article

Pubmed Style

Altındiş M, Cetinkaya Z, Kalaycı R, Ciftci �H, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbiol Infect Dis. 2011; 1(1): 5-9 .

doi:10.5799/jmid2011.01.0002

Web Style

Altındiş M, Cetinkaya Z, Kalaycı R, Ciftci �H, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. https://www.jmidonline.org/?mno=302657098 [Access: June 27, 2026].

doi:10.5799/jmid2011.01.0002

AMA (American Medical Association) Style

Altındiş M, Cetinkaya Z, Kalaycı R, Ciftci �H, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbiol Infect Dis. 2011; 1(1): 5-9 .

doi:10.5799/jmid2011.01.0002

Vancouver/ICMJE Style

doi:10.5799/jmid2011.01.0002

Harvard Style

Altındiş, M., Cetinkaya, . Z., Kalaycı, . R., Ciftci, . �. H., Arslan, . A. & Aktepe, . O. C. (2011) Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbiol Infect Dis, 1 (1), 5-9 .

doi:10.5799/jmid2011.01.0002

Turabian Style

Altındiş, Mustafa, Zafer Cetinkaya, Raike Kalaycı, İhsan H. Ciftci, Alpaslan Arslan, and Orhan C. Aktepe. 2011. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. Journal of Microbiology and Infectious Diseases, 1 (1), 5-9 .

doi:10.5799/jmid2011.01.0002

Chicago Style

Altındiş, Mustafa, Zafer Cetinkaya, Raike Kalaycı, İhsan H. Ciftci, Alpaslan Arslan, and Orhan C. Aktepe. "Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs." Journal of Microbiology and Infectious Diseases 1 (2011), 5-9 .

doi:10.5799/jmid2011.01.0002

MLA (The Modern Language Association) Style

doi:10.5799/jmid2011.01.0002

APA (American Psychological Association) Style

doi:10.5799/jmid2011.01.0002

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