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2024, Vol: 14, Issue: 4
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Research Article 


Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa.


Cited By:2

Abstract
Objectives:Echinococcosis is a cyclozoonotic disease caused by E. granulosus. The canine mainly the dog represents the definitive host and immunity plays an important role in the control of the disease. This study was aimed to detect Echinococcus granulosus B-antibody for Dot-ELISA and antigen for counter-current immune electrophoresis (CIEP) and bacterial co-agglutination assay (Co-A).
Methods: Follow-up of 23 surgical and chemotherapy patients from different hospitals of Khartoum were performed.
Results: Results: Sensitivity and specificity for Dot-ELISA was 94.4% and 100%, respectively within seven days to 6 months except for the relapse all or most of the cases were negative for the disease whereas the sensitivity of CIEP and Co-A for Echinococcusgranulosus antigen in patient sera was 77.8% and 94.4% respectively with a specificity of 100% and 75% respectively. Dot-ELISA, showed high positive and negative predictive value (100%, 95.2%), respectively with CIEP (100%, 83.3%) and in Co-A (81%, 94.1%).
Conclusion: Conclusion: Therefore, detection of antigen of E. granulosus in the serum of operative, post-operative, or chemotherapeutic treated patient using CIEP and Co-A or detection of antibodies using IgG ELISA to the antigen B-rich fraction in (ELISA) assay is useful for following treated hydatid patients. J Microbiol Infect Dis 2021; 11(2):88-94.

Key words: Echinococcus granulosus, Dot-ELISA, Co-agglutination assay, immune electrophoresis


 
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How to Cite this Article
Pubmed Style

Elamin OHS, Ali MA, Elgezuli IAA, Bashir MA, Kahwa I. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. 2021; 11(2): 88-94 . doi:10.5799/jmid.951600

Web Style

Elamin OHS, Ali MA, Elgezuli IAA, Bashir MA, Kahwa I. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. https://www.jmidonline.org/?mno=302657119 [Access: January 09, 2025]. doi:10.5799/jmid.951600

AMA (American Medical Association) Style

Elamin OHS, Ali MA, Elgezuli IAA, Bashir MA, Kahwa I. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. 2021; 11(2): 88-94 . doi:10.5799/jmid.951600


Vancouver/ICMJE Style

Elamin OHS, Ali MA, Elgezuli IAA, Bashir MA, Kahwa I. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. (2021), [cited January 09, 2025]; 11(2): 88-94 . doi:10.5799/jmid.951600


Harvard Style

Elamin, O. H. S., Ali, . M. A., Elgezuli, . I. A. A., Bashir, . M. A. & Kahwa, . I. (2021) Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis, 11 (2), 88-94 . doi:10.5799/jmid.951600


Turabian Style

Elamin, Omer Hassan Siddig, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, and Ivan Kahwa. 2021. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases, 11 (2), 88-94 . doi:10.5799/jmid.951600


Chicago Style

Elamin, Omer Hassan Siddig, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, and Ivan Kahwa. "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination." Journal of Microbiology and Infectious Diseases 11 (2021), 88-94 . doi:10.5799/jmid.951600


MLA (The Modern Language Association) Style

Elamin, Omer Hassan Siddig, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, and Ivan Kahwa. "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination." Journal of Microbiology and Infectious Diseases 11.2 (2021), 88-94 . Print. doi:10.5799/jmid.951600


APA (American Psychological Association) Style

Elamin, O. H. S., Ali, . M. A., Elgezuli, . I. A. A., Bashir, . M. A. & Kahwa, . I. (2021) Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases, 11 (2), 88-94 . doi:10.5799/jmid.951600


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