eISSN : 2146-9369
Creative Commons Lisansı
Journal of Clinical and Experimental Investigations is licenced under the terms of Creative Commons Attribution-Non Commercial4.0 International (CC BY-NC 4.0) License.
Abstract - Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination
Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa

Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination

Omer Hassan Siddig Elamin1, Mai Abdalla Ali2, Ibrahim Alhag Almahadi Elgezuli3, Mona Alrasheed Bashir4, Ivan Kahwa5,6

1Department of Microbiology., Faculty of Medicine, University of Khartoum, Sudan

2Department of Biochemistry and Molecular Biology, Faculty of Science, University of Gezira, Sudan

3Faculty of Health and Environmental Sciences, University of Gezira, Sudan

4Department of Biotechnology, Faculty of Science and Technology, Omdurman Islamic University, Sudan

5Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda

6Pharm-Biotechnology and Traditional Medicine Centre of Excellence (ACEII), Mbarara, Uganda

 

ABSTRACT

Objectives: Echinococcosis is a cyclozoonotic disease caused by E. granulosus. The canine mainly the dog represents the definitive host and immunity plays an important role in the control of the disease. This study was aimed to detect Echinococcus granulosus B-antibody for Dot-ELISA and antigen for counter-current immune electrophoresis (CIEP) and bacterial co-agglutination assay (Co-A).

Methods: Follow-up of 23 surgical and chemotherapy patients from different hospitals of Khartoum were performed.

Results: Results: Sensitivity and specificity for Dot-ELISA was 94.4% and 100%, respectively within seven days to 6 months except for the relapse all or most of the cases were negative for the disease whereas the sensitivity of CIEP and Co-A for Echinococcus granulosus antigen in patient sera was 77.8% and 94.4% respectively with a specificity of 100% and 75% respectively. Dot-ELISA, showed high positive and negative predictive value (100%, 95.2%), respectively with CIEP (100%, 83.3%) and in Co-A (81%, 94.1%).

Conclusion: Conclusion: Therefore, detection of antigen of E. granulosus in the serum of operative, post-operative, or chemotherapeutic treated patient using CIEP and Co-A or detection of antibodies using IgG ELISA to the antigen B-rich fraction in (ELISA) assay is useful for following treated hydatid patients. J Microbiol Infect Dis 2021; 11(2):88-94.

Keywords: Echinococcus granulosus, Dot-ELISA, Co-agglutination assay, immune electrophoresis

Volume 11, Number 02 (2021)